Journal: International Journal of Molecular Sciences

Article Title: Long-Chain Acylcarnitines Decrease the Phosphorylation of the Insulin Receptor at Tyr1151 Through a PTP1B-Dependent Mechanism

doi: 10.3390/ijms22126470

Figure Lengend Snippet: Effects of palmitoylcarnitine (PC) on insulin-producing RIN5F cells. Insulin release in the presence of PC ( A ). The insulin secretion in the media was compared by normalizing the insulin concentration to that of the control group incubated without PC. The graph shows the relative insulin concentration compared to the control group treated without PC. Insulin release was measured 1 h after the cell growth media was changed to DMEM high glucose media (11 mM glucose) containing various concentrations of PC. Representative bands from RIN5F cells ( B ). Semiquantitative data showing relative Akt Ser473 phosphorylation ( C ). The phosphorylation of Akt at Ser473 in RIN5F cells was determined using WB after a 1 h incubation with various concentrations of PC. The band intensities were normalized by dividing each value by the intensity of the total Akt band. The band intensities of the control were set to 1. The results are presented as the means ± standard deviation. * p < 0.05 compared to the control group (one-way ANOVA, Tukey’s multiple comparisons test). Sample size of the groups in section A: control n = 4, PC 1 µM n = 4, PC 5 µM n = 4, and PC 10 µM n = 4. Sample size of the groups in section C: control n = 9, PC 10 µM n = 9.

Article Snippet: Twenty-four hours before the assay, the media were changed to DMEM high glucose (cat. D1152, Merck KGaA) without FBS.

Techniques: Concentration Assay, Control, Incubation, Phospho-proteomics, Standard Deviation